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KMID : 1005220120270030127
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Journal of Embryo Transfer
2012 Volume.27 No. 3 p.127 ~ p.131
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Effects of Parity and Season on Production of Embryos in Superovulated Hanwoo
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Song Sang-Hyun
Jang Duk-Il
Min Chan-Sik
Park Jun-Kyu
Joo Young-Kuk
Lee Jung-Gyu
Jung Ki-Hwa
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Abstract
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This study was performed to investigate the effects of parity and season on the embryo production in superovulated Hanwoo cows. Superovulation was performed from 1 to 8 times by repeated superovulation treatment of Hanwoo cows (n = 22). Irrespective of estrous cycle, donor cows were received a CIDR, progesterone (50 mg) and estradiol benzoate (2.5 mg). After 4.5 days, the donor cows were superovulated with total 28AU FSH (Antorin R-10) administrated twice daily in a decreasing dose for 4 days. On 6th and 7th of FSH injection, 2.5 mg and 15 mg PGF2α were injected i.m, respectively. CIDR was removed at the 7th FSH injection. The donor cows received 200 μgGnRH at 48 hrs after 1st PGF2α injection. The donor cows were artificially inseminated three times after estrous detection at 12 hr intervals and embryos were recovered 7 days after estrous detection. The mean number of total ova, transferrable embryos, degenerated embryos and unfertilized oocytes were 11.6 ± 7.9, 5.5 ± 4.4, 3.0 ± 3.3 and 2.6 ± 4.1 per donor cows, respectively. A higher number of total ova were recovered in parity 3～5 (14.3 ± 1.3) than 1～2 (8.9 ± 1.9, P<0.05). The number of recovered normal embryos is significantly higher in parity 3～5 (7.3 ± 0.8) than that of over 6 (3.7 ± 1.5). Significantly higher number of total ova and normal embryos were recovered in summer (16.4 ± 2.3, 8.1 ± 1.4) than in autumn (10.1 ± 1.8, 4.5 ± 1.1) and winter (6.3 ± 1.8, 3.3 ± 1.1), respectively (P<0.05). Transferable embryos were significantly higher in summer (7.6 ± 1.3) than in winter (3.0 ± 1.0, P< 0.05). The results were showed that parity and season affecting on the production of embryos in superovulated Hanwoo.
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KEYWORD
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Hanwoo superovulation , parity , season , embryo production
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